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Image Search Results
Journal: Frontiers in Pharmacology
Article Title: Positive Effect of Andrographolide Induced Autophagy on Random-Pattern Skin Flaps Survival
doi: 10.3389/fphar.2021.653035
Figure Lengend Snippet: The upregulation of angiogenesis in the skin flaps via Andro (A,C) IHC of Cadherin5 and VEGF in both groups of ischemic skin flaps (original magnification, ×200; scan bar, 50 μm). (B,D) The total absorbance of Cadherin5 and VEGF in IHC. (E) The results of immunoblotting i.e., the expressions of cadherin 5, MMP9, VEGF in the control as well as Andro groups. (F–H) The quantification of Cadherin5, MMP9, and VEGF expressions in the flaps by measuring their optical densities. The obtained data were presented as means ± SEM. Significance: * p -value < 0.05 and ** p -value < 0.01, vs. control group (n = 6 per group).
Article Snippet: Diaminobenzidine (DAB) developer, pentobarbital sodium, and the H&E Kit were procured from Solarbio Science and Technology (Beijing, China), while
Techniques: Western Blot, Control
Journal: Frontiers in Pharmacology
Article Title: Positive Effect of Andrographolide Induced Autophagy on Random-Pattern Skin Flaps Survival
doi: 10.3389/fphar.2021.653035
Figure Lengend Snippet: 3MA reversed the effects of Andro on random-pattern skin flap viability (A) The immunofluorescence data of LC3II in the ischemic skin flap (scan bar, 50 μm). (B) T he histogram representing the percentage of the LC3II-positive cells. (C,E) The immunoblotting of the autophagy associated proteins VPS34, p62, LC3II, Beclin 1, and CTSD; angiogenesis associated proteins VEGF, cadherin5, and MMP9; the oxidative stress-related proteins i.e., SOD1, HO1, and eNOS and the apoptosis-associated proteins i.e., Bax, CYC, and CASP3. (D,F) The quantification of autophagy-, angiogenesis, and oxidative stress-related proteins by measuring their optical densities, as previously mentioned. The obtained data were presented as means ± SEM. Significance: * p -value < 0.05 and ** p -value < 0.01, vs. control group (n = 6 per group).
Article Snippet: Diaminobenzidine (DAB) developer, pentobarbital sodium, and the H&E Kit were procured from Solarbio Science and Technology (Beijing, China), while
Techniques: Immunofluorescence, Western Blot, Control